Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose‐, time‐, and MOI‐dependent manners in human lung epithelial cells A549
Identifieur interne : 000F71 ( Main/Exploration ); précédent : 000F70; suivant : 000F72Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose‐, time‐, and MOI‐dependent manners in human lung epithelial cells A549
Auteurs : Liqi Wu [République populaire de Chine] ; Jianping Dai [République populaire de Chine] ; Xiangfeng Zhao [République populaire de Chine] ; Youying Chen [République populaire de Chine] ; Gefei Wang [République populaire de Chine] ; Kangsheng Li [République populaire de Chine]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2015-07.
Descripteurs français
- KwdFr :
- Antipaludiques (pharmacologie), Antiviraux (pharmacologie), Cellules cultivées, Cellules épithéliales (virologie), Chloroquine (pharmacologie), Effet cytopathogène viral (), Facteurs temps, Humains, Lignée cellulaire tumorale, Relation dose-effet des médicaments, Réplication virale (), Sous-type H1N1 du virus de la grippe A (), Sous-type H1N1 du virus de la grippe A (physiologie).
- MESH :
- pharmacologie : Antipaludiques, Antiviraux, Chloroquine.
- physiologie : Sous-type H1N1 du virus de la grippe A.
- virologie : Cellules épithéliales.
- Cellules cultivées, Effet cytopathogène viral, Facteurs temps, Humains, Lignée cellulaire tumorale, Relation dose-effet des médicaments, Réplication virale, Sous-type H1N1 du virus de la grippe A.
English descriptors
- KwdEn :
- Antimalarials (pharmacology), Antiviral Agents (pharmacology), Cell Line, Tumor, Cells, Cultured, Chloroquine (pharmacology), Cytopathogenic Effect, Viral (drug effects), Dose-Response Relationship, Drug, Epithelial Cells (virology), Humans, Influenza A Virus, H1N1 Subtype (drug effects), Influenza A Virus, H1N1 Subtype (physiology), Time Factors, Virus Replication (drug effects).
- MESH :
- chemical , pharmacology : Antimalarials, Antiviral Agents, Chloroquine.
- drug effects : Cytopathogenic Effect, Viral, Influenza A Virus, H1N1 Subtype, Virus Replication.
- physiology : Influenza A Virus, H1N1 Subtype.
- virology : Epithelial Cells.
- Cell Line, Tumor, Cells, Cultured, Dose-Response Relationship, Drug, Humans, Time Factors.
Abstract
Anti‐malaria drug, chloroquine, has been reported to be effective against influenza A virus (IAV) in vitro and used in in‐vivo experiments and clinical trial for prevention or treatment of influenza. In this study, it has been shown by immunofluorescence, hemagglutination, and plaque assays that chloroquine enhanced A/WSN/33 (H1N1) replication with pronounced cytopathic effect in dose‐, time‐, and MOI‐dependent manners in human lung epithelial cells A549. Time‐of‐addition assay showed that inhibitory effect on virus replication by chloroquine pre‐treatment was indistinctive, and virus productions were enhanced when the drug was applied after viral adsorption. The effectiveness of chloroquine as an anti‐influenza drug is questioned, and caution in its use is recommended. J. Med. Virol. 87:1096–1103, 2015. © 2015 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/jmv.24135
Affiliations:
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qualifier="pharmacology" xml:lang="en"><term>Antimalarials</term><term>Antiviral Agents</term><term>Chloroquine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cytopathogenic Effect, Viral</term><term>Influenza A Virus, H1N1 Subtype</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antipaludiques</term><term>Antiviraux</term><term>Chloroquine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Cells, Cultured</term><term>Dose-Response Relationship, Drug</term><term>Humans</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Effet cytopathogène viral</term><term>Facteurs temps</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Relation dose-effet des médicaments</term><term>Réplication virale</term><term>Sous-type H1N1 du virus de la grippe A</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Anti‐malaria drug, chloroquine, has been reported to be effective against influenza A virus (IAV) in vitro and used in in‐vivo experiments and clinical trial for prevention or treatment of influenza. In this study, it has been shown by immunofluorescence, hemagglutination, and plaque assays that chloroquine enhanced A/WSN/33 (H1N1) replication with pronounced cytopathic effect in dose‐, time‐, and MOI‐dependent manners in human lung epithelial cells A549. Time‐of‐addition assay showed that inhibitory effect on virus replication by chloroquine pre‐treatment was indistinctive, and virus productions were enhanced when the drug was applied after viral adsorption. The effectiveness of chloroquine as an anti‐influenza drug is questioned, and caution in its use is recommended. J. Med. Virol. 87:1096–1103, 2015. © 2015 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Liqi" sort="Wu, Liqi" uniqKey="Wu L" first="Liqi" last="Wu">Liqi Wu</name></noRegion><name sortKey="Chen, Youying" sort="Chen, Youying" uniqKey="Chen Y" first="Youying" last="Chen">Youying Chen</name><name sortKey="Chen, Youying" sort="Chen, Youying" uniqKey="Chen Y" first="Youying" last="Chen">Youying Chen</name><name sortKey="Dai, Jianping" sort="Dai, Jianping" uniqKey="Dai J" first="Jianping" last="Dai">Jianping Dai</name><name sortKey="Dai, Jianping" sort="Dai, Jianping" uniqKey="Dai J" first="Jianping" last="Dai">Jianping Dai</name><name sortKey="Li, Kangsheng" sort="Li, Kangsheng" uniqKey="Li K" first="Kangsheng" last="Li">Kangsheng Li</name><name sortKey="Li, Kangsheng" sort="Li, Kangsheng" uniqKey="Li K" first="Kangsheng" last="Li">Kangsheng Li</name><name sortKey="Li, Kangsheng" sort="Li, Kangsheng" uniqKey="Li K" first="Kangsheng" last="Li">Kangsheng Li</name><name sortKey="Wang, Gefei" sort="Wang, Gefei" uniqKey="Wang G" first="Gefei" last="Wang">Gefei Wang</name><name sortKey="Wang, Gefei" sort="Wang, Gefei" uniqKey="Wang G" first="Gefei" last="Wang">Gefei Wang</name><name sortKey="Wu, Liqi" sort="Wu, Liqi" uniqKey="Wu L" first="Liqi" last="Wu">Liqi Wu</name><name sortKey="Zhao, Xiangfeng" sort="Zhao, Xiangfeng" uniqKey="Zhao X" first="Xiangfeng" last="Zhao">Xiangfeng Zhao</name><name sortKey="Zhao, Xiangfeng" sort="Zhao, Xiangfeng" uniqKey="Zhao X" first="Xiangfeng" last="Zhao">Xiangfeng Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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